Now showing items 741-760 of 990

    • Apparent cytochrome gene dose effects in F-lac and F-gal heterogenotes of Escherichia coli. 

      Shipp, W.; Piotrowski, M.; Friedman, Alan (Elsevier: Archives of Biochemistry and Biophysics, 1972-06)
      The levels of b- and c-type cytochromes and cytochromes a1 and a2 are elevated in a partial diploid strain of E. coli carrying the F2-gal episome under a variety of different growth conditions. Only the levels of b- and ...
    • Origins of high sequence selectivity: a stopped-flow kinetics study of DNA/RNA hybridization by duplex- and triplex-forming oligonucleotides 

      Wang, Shaohui; Friedman, Alan; Kool, Eric (The American Chemical Society: Biochemistry, 1995-08-01)
      Stopped-flow UV kinetics and thermal denaturation experiments are used to examine the origins of high sequence selectivity and binding affinity of circular triplex-forming oligonucleotides with single-stranded DNA/RNA ...
    • Horseradish peroxidase Phe172-->Tyr mutant. Sequential formation of compound I with a porphyrin radical cation and a protein radical. 

      Miller, Vaughn; Goodin, David; Friedman, Alan; Hartmann, Christa; Ortiz de Montellano, Paul (Waverly: Journal of Biological Chemistry, 1995-08-04)
      A gene coding for the F172Y mutant of horseradish peroxidase isozyme C (HRP) has been constructed and expressed in both Spodoptera frugiperda (SF-9) and Trichoplusia ni egg cell homogenate (HighFive) cells. Homology modeling ...
    • Chemical carcinogen in vitro testing: a method for sizing cell nuclei in the nuclear enlargement assay 

      Finch, Robert; Evans, Irene; Bosmann, H. Bruce (Elsevier: Toxicology, 1980)
      The observation that cells often respond to carcinogens by nuclear enlargement has suggested that this property might be useful to develop a short-term screening test for such compounds. Previous methods for detecting ...
    • Papillomavirus DNA replication 

      Ferran, Maureen; McBride, Alison (Humana Press: Methods in Molecular Medicine, 1999-09)
      Papillomavirus genomes replicate and are maintained as stable extrachromosomal plasmid DNA (episomes) in many cell lines. This process requires the viral E1 and E2 proteins and the origin of replication. The minimal origin ...
    • Repression of HPV16 early region transcription by the E2 protein 

      Soeda, Emiko; Ferran, Maureen; Baker, Carl; McBride, Alison (Elsevier: Virology, 2006-07-20)
      HPV16 DNA is often integrated in cancers, disrupting the E1 or E2 genes. E2 can repress the E6/E7 promoter, but other models have been proposed to explain why integration promotes malignant progression. E1 and E2 are ...
    • NF-κB Activation is delayed in mouse L929 cells infected with interferon suppressing, but not inducing, vesicular stomatitis virus strains 

      Boulares, A. Hamid; Ferran, Maureen; Lucas-Lenard, J. (Elsevier: Virology, 1996-03-01)
      Vesicular stomatitis virus (VSV) mutant T1026R1 of the Indiana (IN) serotype is a good inducer of interferon (IFN). This mutant was used to study the activation of NF-κB, a transcription factor necessary for IFN induction, ...
    • Tree eye endothelial cells undergo apoptosis after serum starvation 

      Evans, Irene; Van Winkle, B. (Association for Research in Vision and Ophthalmology: Investigative Ophthalmology and Visual Science, 2002)
      Purpose: Several explanations have been suggested for why endothelial cells undergo apoptosis in the regressing blood vessels found in the newborn rat eye. These explanations hypothesize that apoptosis occurs due to lack ...
    • Nutrient deprivation may be a poor inducer of apoptosis in the newborn rat eye 

      Evans, Irene; Adams, S. (Association for Research in Vision and Ophthalmology: Investigative Ophthalmology and Visual Science, 2003)
      Purpose: The hyaloid vasculature of the newborn rat eye persists after birth and regresses postnatally. One hypothesis is that regression occurs due to a decrease in blood flow to the hyaloid after retinal development and ...
    • The microarray revolution 

      Brewster, Jay; Beason, K.; Eckdahl, Todd; Evans, Irene (Elsevier: Biochemistry and Molecular Biology Education, 2004-07)
      In recent years, microarray analysis has become a key experimental tool, enabling the analysis of genome-wide patterns of gene expression. This review approaches the microarray revolution with a focus upon four topics: 1) ...
    • Apoptosis of the hyaloid vessels in vivo and in vitro in the newborn rat eye 

      Evans, Irene; Curanovic, D.; Bucher, B.; Roberts, S. (Association for Research in Vision and Ophthalmology: Investigative Ophthalmology and Visual Science, 2004)
      Purpose: In the newborn rat eye, the hyaloid vasculature persists after birth and regresses postnatally. Several studies have shown that apoptosis plays a role in this regression although the mechanism is unknown. Apoptosis ...
    • Visualization of the regression of the hyaloid vasculature and retinal vessel formation in the newborn rat using FITC/Dextran 

      Evans, Irene; Bucher, B.; Ocon, A.; Tapley, J. (Association for Research in Vision and Ophthalmology: Investigative Ophthalmology and Visual Science, 2005)
      Purpose: The regression of the pupillary membrane and hyaloid vasculature of the eye was observed in the newborn rat from postpartum days 1–36 by injection of FITC–dextran. We also observed the development of the retinal ...
    • 5-Bromodeoxyuridine does not affect development of the sea urchin, Arbacia punctulata 

      Evans, Irene; Gross, Paul (Elsevier: Experimental Cell Research, 1978-06)
      Development of Arbacia punctulata embryos in the presence of bromodeoxyuridine (BUdR) was investigated under conditions which allowed about 20% substitution of BUdR for thymidine residues in DNA. Development was essentially ...
    • Confocal imaging of the regression of the rat hyaloid vasculature 

      Evans, Irene; Donahue, Margret (Association for Research in Vision and Ophthalmology: Investigative Ophthalmology and Visual Science, 2006)
      Purpose: Regression of the hyaloid/fetal vessel system was studied in the newborn rat using confocal microscopy. The hyaloid vasculature composed of the hyaloid artery (HA), vasa hyaloidia propia (VHP), tunica vasculosa ...
    • Cleaveable CD40Ig fusion proteins and the binding to sgp39 

      Hollenbaugh, Diane; Douthwright, Jean; McDonald, Vicki; Aruffo, Alejandro (Elsevier: Journal of Immunological Methods, 1995-12-15)
      Recombinant immunoglobulin (Ig) fusion proteins of cell surface and intracellular proteins have wide applications. For example, fusion proteins have been used in the isolation, identification and study of ligands and the ...
    • Rapid, room temperature, high-density hydrogen adsorption on single-walled carbon nanotubes at atmospheric pressure assisted by a metal alloy 

      Heben, Michael; Dillon, Anne; Gennett, Thomas; Alleman, Jeffrey; Parilla, Philip; Jones, K.; Hornyak, G. (Materials Research Society 2000 Fall Meeting: Symposium A: Nanotubes and Related Materials, 2001)
      Laser-generated, carbon single-walled nanotubes (SWNTs) adsorb hydrogen in a matter of minutes at room temperature and atmospheric pressure in the presence of a Ti-6Al-4V metal alloy. The unusual hydrogen adsorption ...
    • Synthesis and characterization of boron-doped single-wall carbon nanotubes produced by the laser vaporization technique 

      Blackburn, Jeff; Yan, Yanfa; Engtrakul, Chaiwat; Parilla, Philip; Jones, Kim; Gennett, Thomas; Dillon, Anne; Heben, Michael (The American Chemical Society: Chemistry of Materials, 2006-05-16)
      We present the first successful high-yield laser vaporization synthesis of high-quality boron-doped single-wall carbon nanotubes (B-SWNTs). Boron was loaded into graphite targets in the form of elemental B as well as nickel ...
    • Single wall carbon nanotube-polymer solar cells 

      Bailey, Sheila; Castro, Stephanie; Landi, Brian; Gennett, Thomas; Raffaelle, Ryne (NASA: Glenn Research Center Technical Reports, 2005)
      Investigation of single wall carbon nanotube (SWNT)-polymer solar cells has been conducted towards developing alternative lightweight, flexible devices for space power applications. Photovoltaic devices were constructed ...
    • Cp3M3(CO)3 clusters: electrocatalytic isomerization and effect of changes in metal and carbon monoxide bonding mode on electron-transfer properties 

      Mevs, Judith; Gennett, Thomas; Geiger, William (The American Chemical Society: Organometallics, 1991-05)
      The electrochemical reduction of three trinuclear complexes of general formula Cp3M3(CO)3 (Cp = n^5-cyclopentadienyl, M = Co or Rh) was studied in nonaqueous solutions. The cobalt complex Cp3CO3(CO)3 (3) is reduced by one ...
    • Fuel cell applications of single wall carbon nanotubes 

      Gennett, Thomas; Raffaelle, Ryne (American Institute of Aeronautics and Astronautics: International Energy Conversion Engineering Conference, 2003-08-17)
      Novel carbon materials with nanometer dimensions are of potentially significant importance for a number of advanced technological applications. Currently, considerable interest exists in the possible applications of single ...