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dc.contributor.authorBloustine, J.en_US
dc.contributor.authorVirmani, T.en_US
dc.contributor.authorThurston, Georgeen_US
dc.contributor.authorFraden, S.en_US
dc.identifier.citationPhysical Review Letters 96N8 (2006) 087803en_US
dc.descriptionRIT community members may access full-text via RIT Libraries licensed databases:
dc.description.abstractMeasurements of liquid-liquid phase transition temperatures (cloud points) of mixtures of a protein (lysozyme) and a polymer, poly(ethylene glycol) (PEG) show that the addition of low molecular weight PEG stabilizes the mixture whereas high molecular weight PEG was destabilizing. We demonstrate that this behavior is inconsistent with an entropic depletion interaction between lysozyme and PEG and suggest that an energetic attraction between lysozyme and PEG is responsible. In order to independently characterize the lysozyme/PEG interactions, light scattering experiments on the same mixtures were performed to measure second and third virial coefficients. These measurements indicate that PEG induces repulsion between lysozyme molecules, contrary to the depletion prediction. Furthermore, it is shown that third virial terms must be included in the mixture's free energy in order to qualitatively capture our cloud point and light scattering data. The light scattering results were consistent with the cloud point measurements and indicate that attractions do exist between lysozyme and PEG.en_US
dc.description.sponsorshipResearch was funded by the NASA Office of Biological & Physical Research, Fundamental Microgravity Research in Physical Sciences (Fluid Physics) Grant # NAG3-2386. G.M.T. was supported by the National Institutes of Health grant # NIH EY 11840.en_US
dc.format.extent148970 bytesen_US
dc.subjectLight scatteringen_US
dc.subjectLiquid-liquid transformationsen_US
dc.subjectMolecular weighten_US
dc.titleLight scattering and phase behavior of lysozyme-PEG mixturesen_US

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