Chemical carcinogen in vitro testing: a method for sizing cell nuclei in the nuclear enlargement assay
dc.contributor.author | Finch, Robert | en_US |
dc.contributor.author | Evans, Irene | en_US |
dc.contributor.author | Bosmann, H. Bruce | en_US |
dc.date.accessioned | 2006-08-14T15:40:27Z | en_US |
dc.date.available | 2006-08-14T15:40:27Z | en_US |
dc.date.issued | 1980 | en_US |
dc.identifier.citation | Toxicology 15N2 (1980) 145-154 | en_US |
dc.identifier.issn | 0300-483X | en_US |
dc.identifier.uri | http://hdl.handle.net/1850/2286 | en_US |
dc.description.abstract | The observation that cells often respond to carcinogens by nuclear enlargement has suggested that this property might be useful to develop a short-term screening test for such compounds. Previous methods for detecting nuclear size increases have used an image analyzer system to detect nuclear changes in individual cells. This paper details a more rapid method for obtaining nuclei by use of a stromalyzing procedure following by analysis of nuclear volumes, using a Coulter Counter Channelyzer. This new and simplified nuclear sizing method should facilitate the use of the assay as a possible carcinogenesis screen by permitting rapid and efficient testing of large numbers of compounds. | en_US |
dc.format.extent | 37365 bytes | en_US |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier: Toxicology | en_US |
dc.subject | Carcinogens | en_US |
dc.subject | Nuclear enlargement | en_US |
dc.subject | Screening | en_US |
dc.title | Chemical carcinogen in vitro testing: a method for sizing cell nuclei in the nuclear enlargement assay | en_US |
dc.type | Article | en_US |
dc.identifier.url | http://dx.doi.org/10.1016/0300-483X(80)90008-6 |
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