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Histochemical and biochemical studies of the hepatopancreas peroxidase of the freshwater crayfish, Cambarus robustus

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dc.contributor.author Merrill, Douglas en_US
dc.contributor.author Reife, R. en_US
dc.contributor.author Tiersch, T. en_US
dc.contributor.author Austin, R. en_US
dc.date.accessioned 2006-08-21T19:50:57Z en_US
dc.date.available 2006-08-21T19:50:57Z en_US
dc.date.issued 1985-05 en_US
dc.identifier.citation Journal of Morphology 184N2 (1985) 171-182 en_US
dc.identifier.issn 1097-4687 en_US
dc.identifier.uri http://hdl.handle.net/1850/2389 en_US
dc.description.abstract Crayfish are among the few invertebrate species reported to possess endogenous peroxidase activity. The enzyme is found within the hepatopancreas, the principal digestive and absorptive organ of the crustacean body. Cambarus robustus, a species found in abundance in the streams of western New York, was used in this study. Homogenates of 18 hepatopancreases were assayed for peroxidase activity using guaiacol as the substrate. Although present in all organs, peroxidase activity displayed a greater than 50-fold difference between the two extremes (0.05-;2.72 units/mg protein). Histochemical examination using diaminobenzidine revealed peroxidase activity within a line of cells extending along the distal two-thirds of the lengths of all hepatopancreatic tubules. The cells function to synthesize the enzyme, sequester it within vacuoles of increasing size, and eventually secrete it into the tubule lumen. Since the tubule is constantly renewed by distal mitotic activity and concomitant proximal exfoliation, this histochemical technique permits not only the examination of the ontogeny of this peroxidase-positive cell line, but also offers additional insight into the mechanism of hepatopancreatic tubule renewal. en_US
dc.format.extent 26196 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Wiley: Journal of Morphology en_US
dc.subject Freshwater crayfish en_US
dc.subject Hepatopancreas en_US
dc.subject Peroxidase en_US
dc.title Histochemical and biochemical studies of the hepatopancreas peroxidase of the freshwater crayfish, Cambarus robustus en_US
dc.type Article en_US
dc.identifier.url http://dx.doi.org/10.1002/jmor.1051840207

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