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Ancient HLA genes from 7,500-year-old archaeological remains

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dc.contributor.author Lawlor, David en_US
dc.contributor.author Dickel, Cynthia en_US
dc.contributor.author Hauswirth, William en_US
dc.contributor.author Parham, Peter en_US
dc.date.accessioned 2006-08-21T19:56:23Z en_US
dc.date.available 2006-08-21T19:56:23Z en_US
dc.date.issued 1991-02-28 en_US
dc.identifier.citation Nature 349 (1991) 785 – 788 en_US
dc.identifier.issn 1476-4679 en_US
dc.identifier.uri http://hdl.handle.net/1850/2410 en_US
dc.description.abstract In the past decade there has been increasing interest in cloning DNA from ancient and preserved tissues1−6. Most studies, however, have focused on mitochondrial or chloroplast genes, present at hundreds to thousands of copies per cell compared with one or two for each nuclear gene7−9. With a probe containing Alu repeat sequences, Pääbo isolated a 3.4-kilobase DNA fragment from a 2,400-year-old Egyptian mummy10 which was subsequently shown to contain an intron of the nuclear gene HLA-DQA (ref. 11). Here we report a more targeted approach to the characterization of nuclear genes from archaeological specimens. The Windover pond of central Florida has provided skeletal and soft tissue remains from 165 humans, radiocarbon-dated to be 6,990−8,130 years old12−14. Using DNA obtained from one individual we have characterized segments from six nuclear genes: that for 2-microglobulin and five members of the class I HLA heavy chain gene family. Distinctive patterns of nucleotide substitution in the cloned heavy chain gene segments permit tentative assignment of the HLA-A,B type of the ancient individual. en_US
dc.format.extent 27604 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Nature Publishing Group: Nature en_US
dc.title Ancient HLA genes from 7,500-year-old archaeological remains en_US
dc.type Article en_US
dc.subject.keyword DNA cloning en_US
dc.subject.keyword Heavy gene chains en_US
dc.subject.keyword Nuclear genes en_US
dc.identifier.url http://dx.doi.org/10.1038/349785a0


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