RIT Digital Archive

Ancient HLA genes from 7,500-year-old archaeological remains

RIT Digital Archive

Show simple item record

dc.contributor.author Lawlor, David en_US
dc.contributor.author Dickel, Cynthia en_US
dc.contributor.author Hauswirth, William en_US
dc.contributor.author Parham, Peter en_US
dc.date.accessioned 2006-08-21T19:56:23Z en_US
dc.date.available 2006-08-21T19:56:23Z en_US
dc.date.issued 1991-02-28 en_US
dc.identifier.citation Nature 349 (1991) 785 – 788 en_US
dc.identifier.issn 1476-4679 en_US
dc.identifier.uri http://hdl.handle.net/1850/2410 en_US
dc.description.abstract In the past decade there has been increasing interest in cloning DNA from ancient and preserved tissues1−6. Most studies, however, have focused on mitochondrial or chloroplast genes, present at hundreds to thousands of copies per cell compared with one or two for each nuclear gene7−9. With a probe containing Alu repeat sequences, Pääbo isolated a 3.4-kilobase DNA fragment from a 2,400-year-old Egyptian mummy10 which was subsequently shown to contain an intron of the nuclear gene HLA-DQA (ref. 11). Here we report a more targeted approach to the characterization of nuclear genes from archaeological specimens. The Windover pond of central Florida has provided skeletal and soft tissue remains from 165 humans, radiocarbon-dated to be 6,990−8,130 years old12−14. Using DNA obtained from one individual we have characterized segments from six nuclear genes: that for 2-microglobulin and five members of the class I HLA heavy chain gene family. Distinctive patterns of nucleotide substitution in the cloned heavy chain gene segments permit tentative assignment of the HLA-A,B type of the ancient individual. en_US
dc.format.extent 27604 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Nature Publishing Group: Nature en_US
dc.title Ancient HLA genes from 7,500-year-old archaeological remains en_US
dc.type Article en_US
dc.subject.keyword DNA cloning en_US
dc.subject.keyword Heavy gene chains en_US
dc.subject.keyword Nuclear genes en_US
dc.identifier.url http://dx.doi.org/10.1038/349785a0

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search RIT Digital Archive

Advanced Search


My Account