| dc.contributor.advisor | Opines | en_US |
| dc.contributor.author | Zhang, Dunhua | en_US |
| dc.contributor.author | Shelby, Richard | en_US |
| dc.contributor.author | Savka, Michael | en_US |
| dc.contributor.author | Dessaux, Yves | en_US |
| dc.contributor.author | Wilson, Mark | en_US |
| dc.date.accessioned | 2006-08-28T20:02:59Z | en_US |
| dc.date.available | 2006-08-28T20:02:59Z | en_US |
| dc.date.issued | 1998-07-17 | en_US |
| dc.identifier.citation | Journal of Chromatography A 813N2 (1998) 247-253 | en_US |
| dc.identifier.issn | 0021-9673 | en_US |
| dc.identifier.uri | http://hdl.handle.net/1850/2526 | en_US |
| dc.description.abstract | Protocols for reversed-phase high-performance liquid chromatography (RP-HPLC) with fluorescence detection were developed for the separation, detection and quantification of imine-linked opines, following 4-fluoro-7-nitrobenzoxadiazole (NBD-F) derivatization. Both natural opines (mannopine, mannopinic acid, cucumopine, octopine, octopinic acid, and nopaline) and opine analogs (nor-mannopine, galactopine, glucopine) were included in the study. The on-column detection limits for NBD-opines varied from 0.1 pmol to 5 pmol. These methods were applied to quantify mannopine on the leaf surfaces of transgenic tobacco (Nicotiana tabacum) plants expressing mannityl opine synthesis (mas) genes from Agrobacterium tumefaciens. | en_US |
| dc.description.sponsorship | This work was supported by a grant to M.W. from the USDA NRIGGP ( No. 9501182). | en_US |
| dc.format.extent | 37365 bytes | en_US |
| dc.format.mimetype | application/pdf | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | Elsevier: Journal of Chromatography | en_US |
| dc.subject | Agrobacteria | en_US |
| dc.subject | Detection | en_US |
| dc.title | Separation, detection, and quantification of imine-linked opines by high-performance liquid chromatography | en_US |
| dc.type | Article | en_US |
| dc.identifier.url | http://dx.doi.org/10.1016/S0021-9673(98)00346-X | |
