RIT Digital Archive

A liquid culture method for rescuing immature embryos of eastern cottonwood

RIT Digital Archive

Show simple item record

dc.contributor.author Savka, Michael en_US
dc.contributor.author Dawson, Jeffrey en_US
dc.contributor.author Jokela, Jalmer en_US
dc.contributor.author Skiryin, Robert en_US
dc.date.accessioned 2006-08-28T20:04:07Z en_US
dc.date.available 2006-08-28T20:04:07Z en_US
dc.date.issued 1987-01 en_US
dc.identifier.citation Plant Cell, Tissue and Organ Culture 10N3 (1987) 221-226 en_US
dc.identifier.issn 1573-5044 en_US
dc.identifier.uri http://hdl.handle.net/1850/2531 en_US
dc.description.abstract A one-step method to rescue immature embryos of eastern cottonwood (Populus deltoides Bartr.) is described. Plantlets developed from 83% of 25-day-old embryos grown in shaken culture on Murashige and Skoog (MS) liquid medium with 2.2 u-m indole-3-acetic acid (IAA) and from 86% of embryos not supplemented with IAA. In contrast, when the MS medium was solidified with 0.8% agar, plantlets developed from 25% of 25-day-old embryos cultured on medium supplemented with IAA and from 28% of embryos in medium not supplemented with IAA. Eighty eight percent of all plantlets survived a gradual acclimitization to peat plugs in a greenhouse. The one-step liquid-culture method is an effective means of rescuing immature embryos by ovule culture from excised artificially-pollinated female branches in our cottonwood breeding program. en_US
dc.format.extent 43151 bytes en_US
dc.format.mimetype application/pdf en_US
dc.language.iso en_US en_US
dc.publisher Springer: Plant Cell, Tissue and Organ Culture en_US
dc.subject Eastern cottonwood en_US
dc.subject Embryo culture en_US
dc.subject Liquid culture en_US
dc.title A liquid culture method for rescuing immature embryos of eastern cottonwood en_US
dc.type Article en_US
dc.identifier.url http://dx.doi.org/10.1007/BF00037306

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search RIT Digital Archive

Advanced Search


My Account