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dc.contributor.authorSavka, Michaelen_US
dc.contributor.authorFarrand, Stephenen_US
dc.date.accessioned2006-08-28T20:05:24Zen_US
dc.date.available2006-08-28T20:05:24Zen_US
dc.date.issued1992-02en_US
dc.identifier.citationPlant Physiology 98N2 (1992) 784-789en_US
dc.identifier.issn1532-2548en_US
dc.identifier.urihttp://hdl.handle.net/1850/2536en_US
dc.description.abstractThree genes from the TR region of pTi15955 were introduced into tobacco (Nicotiana tabacum L.) to direct the synthesis of the mannityl opines from hexose sugars and glutamine or glutamate. Opines were present in all tissue types tested and accumulated to levels of 100 to 150 micrograms per milligram dry weight in root, stem, and leaf tissues. Opine-producing plants appeared normal with respect to morphology and development. Transgenic plants grown for 60 days under sterile autotrophic conditions produced up to 540 micrograms of the mannityl opines per milligrams dry weight of tissue as root exudates. Opines were also detected in leaf and seed washes from soil-grown plants.en_US
dc.description.sponsorshipSupported by grant No. 86-12-77-3 from the Illinois Soybean Program Operating Board to S.K.F.en_US
dc.format.extent38583 bytesen_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherAmerican Society of Plant Biologists: Plant Physiologyen_US
dc.subjectBacterial infectionen_US
dc.subjectOpinesen_US
dc.subjectTobaccoen_US
dc.titleMannityl opine accumulation and exudation by transgenic tobaccoen_US
dc.typeArticleen_US
dc.identifier.urlhttp://dx.doi.org/10.1104/pp.98.2.784


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