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dc.contributor.authorTofani, Lucaen_US
dc.contributor.authorFeis, Alessandroen_US
dc.contributor.authorSnoke, Royen_US
dc.contributor.authorBerti, Deboraen_US
dc.contributor.authorBaglioni, Pieroen_US
dc.contributor.authorSmulevich, Giuliettaen_US
dc.date.accessioned2006-08-29T18:36:02Zen_US
dc.date.available2006-08-29T18:36:02Zen_US
dc.date.issued2004-08en_US
dc.identifier.citationBiophysical Journal 87N2 (2004) 1186-1195en_US
dc.identifier.issn1542-0086en_US
dc.identifier.urihttp://hdl.handle.net/1850/2571en_US
dc.description.abstractThe complexes of horse myoglobin (Mb) with the anionic surfactant sodium dodecyl sulfate (SDS), and with the cationic surfactants cetyltrimethylammonium chloride (CTAC) and decyltrimethylammonium bromide (DeTAB), have been studied by a combination of surface tension measurements and optical spectroscopy, including heme absorption and aromatic amino acid fluorescence. SDS interacts in a monomeric form with Mb, which suggests the existence of a specific binding site for SDS, and induces the formation of a hexacoordinated Mb heme, possibly involving the distal histidine. Fluorescence spectra display an increase of tryptophan emission. Both effects point to an increased protein flexibility. SDS micelles induce both the appearance of two more heme species, one of which has the features of free heme, and protein unfolding. Mb/CTAC complexes display a very different behavior. CTAC monomers have no effect on the absorption spectra, and only a slight effect on the fluorescence spectra, whereas the formation of CTAC aggregates on the protein strongly affects both absorption and fluorescence. Mb/DeTAB complexes behave in a very similar way as Mb/CTAC complexes. The surface activity of the different Mb/surfactant complexes, as well as the interactions between the surfactants and Mb, are discussed on the basis of their structural properties.en_US
dc.description.sponsorshipThis work was supported by the Italian Ministry of Education, University and Research (COFIN 2001031798 (G.S.) and 2003035197 (P.B. and D.B.)) and CSGI (Center for Colloid and Surface Science) (P.B. and D.B.).en_US
dc.format.extent26866 bytesen_US
dc.format.mimetypeapplication/pdfen_US
dc.language.isoen_USen_US
dc.publisherBiophysical Society: Biophysical Journalen_US
dc.subjectMyoglobinen_US
dc.subjectProteinsen_US
dc.subjectSurfactantsen_US
dc.titleSpectroscopic and interfacial properties of myoglobin/surfactant complexesen_US
dc.typeArticleen_US
dc.identifier.urlhttp://dx.doi.org/10.1529/biophysj.104.041731


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