dc.contributor.author | Dietzgen, Ralf | en_US |
dc.contributor.author | Snoke, Roy | en_US |
dc.contributor.author | Babb, Bruce | en_US |
dc.date.accessioned | 2006-08-29T18:37:08Z | en_US |
dc.date.available | 2006-08-29T18:37:08Z | en_US |
dc.date.issued | 1987-08-01 | en_US |
dc.identifier.citation | Analytical Biochemistry 164N2 (1987) 297-302 | en_US |
dc.identifier.issn | 0003-2697 | en_US |
dc.identifier.uri | http://hdl.handle.net/1850/2576 | en_US |
dc.description.abstract | A colorimetric reagent, 4-(4'-nitro-2'-methylsulfonylphenylazo)phenyl phosphate (NMPP), has been shown to be an effective substrate of alkaline phosphatase. NMPP and p-nitrophenyl phosphate were applied in comparative studies using enzyme immunoassays for the detection of viral antigens and antiviral antibodies. The new substrate exhibited similar, or even higher, sensitivity than p-nitrophenyl phosphate depending on the substrate concentrations used. Positive and negative reactions were easier to define, even without cumbersome equipment. The enzyme reaction was terminated by the uncompetitive inhibitor, theophylline. | en_US |
dc.format.extent | 37365 bytes | en_US |
dc.format.mimetype | application/pdf | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier: Analytical Biochemistry | en_US |
dc.subject | Colorimetric reagents | en_US |
dc.subject | Enzyme assays | en_US |
dc.subject | Immunoassays | en_US |
dc.title | An azophenolic colorimetric reagent for use in enzyme-linked immunosorbent assays. | en_US |
dc.type | Article | en_US |
dc.identifier.url | http://dx.doi.org/10.1016/0003-2697(87)90496-9 | |