Portable cell-based biosensor system for toxin detection
Date
1998-12Author
Pancrazio, Joseph
Bey, Paul Jr.
Cuttino, David
Kusel, Julian
Borkholder, David
Shaffer, Kara
Kovacs, Gregory
Stenger, David
Metadata
Show full item recordAbstract
A portable cell-based biosensor has been developed and characterized. The prototype system relies on extracellular recording
from excitable cells cultured over an array of platinized gold microelectrodes. Extracellular potentials were bandpass filtered between 80 Hz to 2.8 kHz and amplified with a selectable gain of either 1000 or 5000. The input-referred noise level of the system was only 8.7 IlVRMS in the laboratory setting, reaching only 10.6 IlVRMS in an outdoor environment, more than sufficient for measurement of extracellular potentials from excitable cells. The system also incorporates a feedback control system for temperature regulation and a 36-channel multiplexer for selection of up to four output channels for simultaneous display. Wherever possible, low-cost 'off-the-shelf components were utilized in this prototype biosensor design. Using this system, extracellular recordings from chick myocardiocytes were performed under both laboratory and outdoor conditions. © 1998
Elsevier Science S.A. All rights reserved.