dc.contributor.author | Pancrazio, Joseph | en_US |
dc.contributor.author | Whelan, J. | en_US |
dc.contributor.author | Borkholder, David | en_US |
dc.contributor.author | Ma, W. | en_US |
dc.contributor.author | Stenger, David | en_US |
dc.date.accessioned | 2008-02-01T20:19:35Z | en_US |
dc.date.available | 2008-02-01T20:19:35Z | en_US |
dc.date.issued | 1999 | en_US |
dc.identifier.citation | Annals of Biomedical Engineering 27 (1999) 697-711 | en_US |
dc.identifier.uri | http://hdl.handle.net/1850/5559 | en_US |
dc.description | RIT community members may access full-text via RIT Libraries licensed databases: http://library.rit.edu/databases/ | |
dc.description.abstract | Biosensors incorporate a biological sensing element that converts a change in an immediate environment to signals conducive for processing. Biosensors have been implemented for a number of applications ranging from environmental pollutant
detection to defense monitoring. Biosensors have two intriguing characteristics: (I) they have a naturally evolved selectivity to biological or biologically active analytes; and (2) biosensors have the capacity to respond to analytes in a physiologically
relevant manner. In this paper, molecular biosensors, based on antibodies, enzymes, ion channels, or nucleic acids, are briefly reviewed. Moreover, cell-based biosensors are reviewed and discussed. Cell-based biosensors have been implemented using microorganisms, particularly for environmental monitoring of pollutants. Biosensors incorporating mammalian cells have a distinct advantage of responding in a manner that can offer insight into the physiological effect of an analyte.
Several approaches for transduction of cellular signals are discussed; these approaches include measures of cell metabolism, impedance, intracellular potentials, and extracellular potentials.
Among these approaches, networks of excitable cells cultured on microelectrodc arrays are uniquely poised to provide rapid, functional classification of an analyte and ultimately constitute a potentially effective cell-based biosensor technology. Three challenges that constitute barriers to increased cell-based biosensor applications are presented: analytical methods, reproducibility, and cell sources. Possible future solutions to these challenges are discussed. © 1999 Biomedical Engineering Society. [S0090-6964(99)O 1406-X] | en_US |
dc.description.sponsorship | This work was supported in part by the Office of Naval Research, the Defense Threat Reduction Agency, U.S. Marine Corps and the Defense Advanced Research Projects Agency. The authors thank Dr. H. J. Bryant (Uniformed Services University of the Health Sciences) and Dr. V. C. Kowtha (NRL) for providing electrophysiological records from NG 108-15 cells, and Dr. P. Manos (Gillette Corp.) for her contribution of spinal cord extracellular recordings. 1n addition, the authors extend their gratitude to Dr. G. P. Anderson and P. T. Charles (NRL)for their discussions on immunosensor technology, and Dr. J. Matthew Mauro (NRL) for his constructive review of this manuscript. The opinions and assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Department of the Navy. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer | en_US |
dc.subject | Antibody | en_US |
dc.subject | Environmental monitoring | en_US |
dc.subject | Functional assay | en_US |
dc.subject | Chemical warfare | en_US |
dc.subject | Extracellular potential | en_US |
dc.subject | Impedance | en_US |
dc.subject | Microelectrode | en_US |
dc.subject | Patterning | en_US |
dc.subject | Stem cells | en_US |
dc.title | Development and application of cell-based biosensors | en_US |
dc.type | Article | en_US |
dc.identifier.url | http://dx.doi.org/10.1114/1.225 | |