A microfluidic cartridge to prepare spores for PCR analysis
Date
2000Author
Belgrader, Phillip
Okuzumi, Margaret
Pourahmadi, Farzad
Borkholder, David
Northrup, M. Allen
Metadata
Show full item recordAbstract
A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores, and dispenses the mixture into PCR trube. The total time to automatically process the spores in the cartridge and then detect the spore DNA by real-time PCR was 20 min.