dc.contributor.author | Jung, D. | en_US |
dc.contributor.author | Cuttino, David | en_US |
dc.contributor.author | Pancrazio, Joseph | en_US |
dc.contributor.author | Manos, Patricia | en_US |
dc.contributor.author | Cluster, T. | en_US |
dc.contributor.author | Sathanoori, R. | en_US |
dc.contributor.author | Aloi, L. | en_US |
dc.contributor.author | Coulombe, M. | en_US |
dc.contributor.author | Czarnaski, M. | en_US |
dc.contributor.author | Borkholder, David | en_US |
dc.contributor.author | Kovacs, Gregory | en_US |
dc.contributor.author | Bey, Paul Jr. | en_US |
dc.contributor.author | Stenger, David | en_US |
dc.contributor.author | Hickman, J. | en_US |
dc.date.accessioned | 2008-02-15T16:54:42Z | en_US |
dc.date.available | 2008-02-15T16:54:42Z | en_US |
dc.date.issued | 1998-05 | en_US |
dc.identifier.citation | Vacuum Science and Technology A 16N3 (1998) 1183-1188 | en_US |
dc.identifier.uri | http://hdl.handle.net/1850/5589 | en_US |
dc.description | RIT community members may access full-text via RIT Libraries licensed databases: http://library.rit.edu/databases/ | |
dc.description.abstract | We are developing a cell-based biosensor consisting of a planar microelectrode array that allows detection of extracellular potentials and their modulation in the presence of toxins or other active agents. To improve cell–electrode coupling, the microelectrodes were electroplated with platinum black. We report on the use of imaging x-ray photoelectron spectroscopy ~XPS!, scanning electron microscopy ~SEM!, impedance measurements, and extracellular recordings to assess the effectiveness of this procedure. SEM provided highly detailed images of the shape and structure of well-formed deposits of thickness on the order of 1 mm or more. Because of its inherent high surface sensitivity, imaging XPS could reveal the presence of platinum deposits that were too thin to be detected by SEM. For typical, well-plated microelectrodes, impedance measurements showed reductions in the electrical resistance at 100 Hz from roughly 60 MV or more 1 MV. The overall electronic coupling of biopotentials to the microelectrodes was demonstrated by recordings obtained from beating rat myocytes and from rat spinal cord cells. | en_US |
dc.description.sponsorship | The authors acknowledge important guidance from Dr. Leslie Tung, Department of Biomedical Engineering, Johns Hopkins University. They thank Rachel Harrison of Johns Hopkins University for demonstrating how to culture the myocytes, Tom Schneider for helpful discussions, and Heather Schessler for preparing the PEDA monolayers. The study of myocytes was funded by the internal research and development program at the Science Applications International Corporation. The study of the microelectrode arrays was funded by the Defense Advanced Research Projects Agency and the Naval Research Laboratory under Contract No. N0001 495-C-2128. The fabrication of the arrays was performed under subcontract by the Integrated Circuits Laboratory, Department of Electrical Engineering, Stanford University. The opinions and assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Department of the Navy. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | American Vacuum Society | en_US |
dc.title | Cell-based sensor microelectrode array characterized by imaging x-ray photoelectron spectroscopy, scanning electron microscopy, impedance measurements, and extracellular recordings | en_US |
dc.type | Article | en_US |
dc.subject.keyword | Microelectrode array | en_US |
dc.subject.keyword | Biopotentials | en_US |
dc.subject.keyword | Biosensors | en_US |
dc.subject.keyword | Platinization | en_US |
dc.identifier.url | http://dx.doi.org/10.1116/1.581256 | |