Separation, detection, and quantification of imine-linked opines by high-performance liquid chromatography
Date
1998-07-17Author
Zhang, Dunhua
Shelby, Richard
Savka, Michael
Dessaux, Yves
Wilson, Mark
Metadata
Show full item recordAbstract
Protocols for reversed-phase high-performance liquid chromatography (RP-HPLC) with fluorescence detection were developed for the separation, detection and quantification of imine-linked opines, following 4-fluoro-7-nitrobenzoxadiazole (NBD-F) derivatization. Both natural opines (mannopine, mannopinic acid, cucumopine, octopine, octopinic acid, and nopaline) and opine analogs (nor-mannopine, galactopine, glucopine) were included in the study. The on-column detection limits for NBD-opines varied from 0.1 pmol to 5 pmol. These methods were applied to quantify mannopine on the leaf surfaces of transgenic tobacco (Nicotiana tabacum) plants expressing mannityl opine synthesis (mas) genes from Agrobacterium tumefaciens.