Browsing Citations Only by Author "Borkholder, David"

Now showing items 1-8 of 8

    • Cell-based sensor microelectrode array characterized by imaging x-ray photoelectron spectroscopy, scanning electron microscopy, impedance measurements, and extracellular recordings 

      Jung, D.; Cuttino, David; Pancrazio, Joseph; Manos, Patricia; Cluster, T.; Sathanoori, R.; Aloi, L.; Coulombe, M.; Czarnaski, M.; Borkholder, David; Kovacs, Gregory; Bey, Paul Jr.; Stenger, David; Hickman, J. (American Vacuum Society, 1998-05)
      We are developing a cell-based biosensor consisting of a planar microelectrode array that allows detection of extracellular potentials and their modulation in the presence of toxins or other active agents. To improve ...

    • Description and demonstration of a CMOS amplifier-based-system with measurement and stimulation capability for bioelectrical signal transduction 

      Pancrazio, Joseph; Bey, Paul Jr.; Loloee, Arash; Manne, SubbaRao; Chao, Hui-Chuan; Howard, Lorn; Gosney, W. Milton; Borkholder, David; Kovacs, Gregory; Manos, Patricia; Cuttino, David; Stenger, David (Elsevier www.elsevier.com/locate/bios, 1998-10)
      An extracellular recording system incorporating an electrode array and an amplifier/stimulator CMOS chip is described and characterized. Important features of this custom VLSI chip include 16 instrumentation amplifiers ...

    • Development and application of cell-based biosensors 

      Pancrazio, Joseph; Whelan, J.; Borkholder, David; Ma, W.; Stenger, David (Springer, 1999)
      Biosensors incorporate a biological sensing element that converts a change in an immediate environment to signals conducive for processing. Biosensors have been implemented for a number of applications ranging from ...

    • Microchip isoelectric focusing using a miniature scanning detection system 

      Raisi, Fariba; Belgrader, Phillip; Borkholder, David; Herr, Amy; Kintz, Gregory; Pourahmadi, Farzad; Taylor, Michael; Northrup, M. Allen (John Wiley & Sons Ltd., 2001)
      A miniature scanning fluorescent detector has been developed for plastic microchannel isoelectric focusing (mIEF) analysis. The detector, comprised of a lamp and photomultiplier tube (PMT) on a moving stage, measured the ...

    • Microelectrode arrays for stimulation of neural slice preparations 

      Borkholder, David; Bao, J.; Maluf, Nadim; Perl, E.R.; Kovacs, Gregory (Elsevier, 1997-11)
      A planar 6 x 6 array of iridium electrodes with four reference electrodes has been developed for use with neural tissue preparations. Precise knowledge of the relative locations of the array elements allows for spatial ...

    • A microfluidic cartridge to prepare spores for PCR analysis 

      Belgrader, Phillip; Okuzumi, Margaret; Pourahmadi, Farzad; Borkholder, David; Northrup, M. Allen (Elsevier www.elsevier.com/locate/bios, 2000)
      A prototype cartridge system is described that rapidly disrupts Bacillus spores by sonication, adds PCR reagent to the disrupted spores, and dispenses the mixture into PCR trube. The total time to automatically process the ...

    • Portable cell-based biosensor system for toxin detection 

      Pancrazio, Joseph; Bey, Paul Jr.; Cuttino, David; Kusel, Julian; Borkholder, David; Shaffer, Kara; Kovacs, Gregory; Stenger, David (Elsevier www.elsevier.com/locate/sensorb, 1998-12)
      A portable cell-based biosensor has been developed and characterized. The prototype system relies on extracellular recording from excitable cells cultured over an array of platinized gold microelectrodes. Extracellular ...

    • Quarter-micron lithography with a gapped Markle-Dyson system 

      Owen, G.; Borkholder, David; Knorr, C.; Markle, David; Pease, R. (American Vacuum Society, 1994-11)
      0.25-µm lithography has previously been demonstrated using an ungapped prototype Markle–Dyson system, in which the wafer was held in soft contact with the mask. However, such an in-contact scheme would be inappropriate for ...